【摘要】Foot-and-mouth disease virus(FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome-and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1(Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication.
【关键词】
《建筑知识》 2015-05-12
《中国医疗管理科学》 2015-05-12
《中国医疗管理科学》 2015-05-12
《中国医疗管理科学》 2015-05-12
《中国果菜》 2015-07-08
《当代体育科技》 2015-07-08
《重庆高教研究》 2015-06-25
《重庆高教研究》 2015-06-30
Copyright © 2013-2016 ZJHJ Corporation,All Rights Reserved
发表评论
登录后发表评论 (已发布 0条)点亮你的头像 秀出你的观点