Lnc-RP5 Regulates the mi R-129-5p/Notch1/PFV Internal Promoter Axis to Promote the Expression of the Prototype Foamy Virus Transactivator Tas

更新时间:2023-05-28

【摘要】Prototype foamy virus(PFV) is a unique retrovirus that infects animals and humans and does not cause clinical symptoms. Long noncoding RNAs(lncRNAs) are believed to exert multiple regulatory functions during viral infections. Previously, we utilized RNA sequencing(RNA-seq) to characterize and identify the lncRNA lnc-RP5-1086 D14.3.1-1:1(lnc-RP5), which is markedly decreased in PFV-infected cells. However, little is known about the function of lnc-RP5 during PFV infection. In this study, we identified lnc-RP5 as a regulator of the PFV transcriptional transactivator(Tas). Lnc-RP5 enhanced the activity of the PFV internal promoter(IP). The expression of PFV Tas was found to be promoted by lnc-RP5. Moreover, mi R-129-5 p was found to be involved in the lnc-RP5-mediated promotion of PFV IP activity, while the Notch1 protein suppressed the activity of PFV IP and the expression of Tas.Our results demonstrate that lnc-RP5 promotes the expression of PFV Tas through the miR-129-5 p/Notch1/PFV IP axis. This work provides evidence that host lnc RNAs can manipulate PFV replication by employing mi RNAs and proteins during an early viral infection.

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